Coding

Part:BBa_K3757006:Design

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-26)


c_KR-12_6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A His6-tag has been embedded into loop 5 and the AMP KR-12 in loop 6. Includes Esp3I (Type IIS restriction enzyme) recognition sites for fast cloning and expression as a cyclic peptide in Nicotiana benthamiana.


Source

MCoTI-II from Momordica cochinchinensis, amino acid sequence found in Cybase (http://www.cybase.org.au/index.php?page=card&table=protein&id=3), reverse transcibed into DNA sequence with codon usage optimized for Nicotiana bethaniana. KR-12 is a fragment of the cathelicidin LL-37 from Homo sapiens, amino acid sequence was found on DBAASP ID: 146 and was also reverse transcribed into DNA sequence.

References